| Date | Home | Away | Result |
|---|---|---|---|
| 28/10/25 19:30 | Frosinone Calcio | Virtus Entella | 4-0 |
| 28/10/25 19:30 | FC Cesena | Carrarese Calcio 1908 | 2-1 |
| 28/10/25 19:30 | FC Empoli | Sampdoria Genua | 1-1 |
| 28/10/25 19:30 | Pescara Calcio | US Avellino | 1-1 |
| 28/10/25 19:30 | AC Reggiana 1919 | Modena FC | 1-0 |
| 28/10/25 19:30 | Palermo FC | AC Monza | 0-3 |
| Cancelled | Juve Stabia | SSC Bari | --- |
| 29/10/25 19:30 | Mantova 1911 | US Catanzaro 1929 | 1-3 |
| 29/10/25 19:30 | Spezia Calcio | Padova Calcio | 1-1 |
| 29/10/25 19:30 | FC Venice | FC Südtirol | 3-0 |